Alessandro Guardassoni (13 December 1819 in Bologna – 1 March 1888 in Bologna) was an Italian painter. He mainly painted religious themes. He trained in the Accademia Pontificia di Belle Arti of Bologna, under Clemente Alberi. He collaborated with Cesare Mauro Trebbi. He painted some of the frescoes in the church of San Giuliano, Bologna. He was named to a professorship of the Accademia Felsinea.